The biofilm formation triggered by uncontrolled protein adsorption, on medical devices is the leading cause of catheter-associated urinary tract infections (CAUTI) during implantation. Herein, we report a simple and one-step strategy to functionalize surfaces of silicone catheters, poly(dimethylsiloxane) (PDMS), with antifouling and antimicrobial substances.. A novel formulation consisting of an anionic polysaccharide (hyaluronic acid, HA) and a lysine-derived biocompatible cationic surfactant (N-ε -myristoyl-lysine methyl ester, MKM) was prepared, resulting in the formation of nanoparticles (NPs, ca. 100 - 250 nm). Besides their high stability and long-lasting hydrophilicity in ambient and aqueous environments for 60 days, the nanometric layers (58 ± 2 nm) of HA-MKM NPs on the PDMS showed very low protein adsorption against BSA and lysozyme and significantly lower adsorption against fibrinogen as revealed by a quartz crystal microbalance with dissipation. In-vitro antimicrobial test with <i>S. Aureus, E. Coli, P. Aeruginosa, P, Mirabilis C. Albicans</i> microbes under dynamic conditions revealed that the microbes’ attachment was hampered by 85% when compared with the unmodified PDMS. Given the multiple functionalities and different physiochemical properties of polysaccharide-lysine-based surfactant mixtures, this approach can be easily extended for the development of novel coatings on other silicone-based materials, thereby broadening potential applicability of PDMS-based devices in microfluidics, diagnostic biosensors and others.<br/>

Bioactive nano-coatings from hyaluronic acid and a lysine-derived surfactant

253rd National Meeting (2017)

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