Bacteria-mediated diseases are global healthcare concern due to the accelerated emergence and spread of drug resistant bacterial strains. Cationic compounds are considered membrane active biocidal agents having a great potential to control bacterial infections while limiting the emergence of drug resistance. Herein, the versatility and simplicity of the Layer-by-Layer (LbL) technique was used to functionalize poly (methyl vinyl ether-co-maleic anhydride) nanoparticles with a highly antibacterial aminocellulose conjugate in a multilayer fashion. The assembly of cationic bearing aminocellulose with negatively charged hyaluronic acid on the particles surface was confirmed by the alternations of their surface charge and size after each deposition step. Stable polyelectrolyte-decorated particles with an average size of 600 nm and zeta potential of ± 40 mV were developed after five LbL assembly cycles. The antibacterial properties of these particles against <i>S. aureus</i> were significantly improved when the polycationic aminocellulose was applied as a top layer. The large number of amino groups available on the particles surface allows for the interaction with intrinsically anionic bacterial cell wall leading to irreparable membrane damage and complete eradication of <i>S. aureus</i> after 24 h treatment, while the hyaluronic acid improve their biocompatibility. This specific mechanism of action is believed to diminish the possibility for selection of new resistant strains and is a great promise in controlling bacterial contamination.

Antibacterial layer-by-layer coating of nanoparticles

253rd National Meeting (2017)

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