Fatty acids are essential components of infant nutrition. The arachadonic acid (AA) and decosahexanoic acid (DHA) are currently attracting a considerable amount of attention due to their pivotal role in development. DHA and AA were important constituents of neuronal membranes and blood vessels in the brain. Fatty acid methyl esters were prepared with sodium methylate and methanolic boron trifluoride and extracted into isooctane following the method modified by AOAC 991.39. Quantitative analysis of the fatty acids was conducted by GC equipped with a 30 m x 0.25mm i.d. HP-FFAP fused silica capillary column and a FID. The uncertainty provided with the value was an expanded uncertainty about the mean to cover the measurand with approximately 95% confidence. The expanded uncertainty was calculated as U=kuc, where uc represents the combined uncertainty, consistent with ISO guide, and k was a coverage factor corresponding to approximately 95% confidence. This method provided good correlation (r&lt;0.9995) and accuracy (&lt;10.2 %) within the test ranges. Contents and expanded uncertainty of linoleic acid, arachidonic acid, alpha-linolenic acid, gamma-linolenic acid, oleic acid, DHA, EPA and stearic acid were 47346.91±3614.69 mg/kg (k=2), 597.88±18.49 mg/kg (k=2), 3787.98±162.11 mg/kg (k=2), 213.01±7.84 mg/kg (k=2), 57054.94±3088.44 mg/kg (k=2), 637.72±45.38 mg/kg (k=2), 173.19±4.83 mg/kg (k=2) and 9949.12±291.59 mg/kg (k=2), respectively.

Fatty acids contents and expanded uncertainty of infant formulas by gas chromatography

254th National Meeting (2017)

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