Identification of damage inflicted on cellular biomolecules is the first step in understanding and facilitating the cellular stress tolerance responses. Although stress induced damage to proteins and DNA is well documented, information about RNA damage is largely extrapolated from studies on DNA. Our initial goal is to document the profile of UVR-induced RNA photoproducts and understand their characteristic production by LC-MS analysis, so that a library of RNA-photoproducts can be developed. Such a library will be helpful for conducting the comparative analysis of differential generation of RNA photoproducts especially when RNA is exposed to UVR with altered genetic background. Systematic studies indicating the appearance of significant amounts of RNA photoproducts, and loss of a specific class of modified nucleosides upon UVR exposure to RNA outside the cell will be presented. These changes will be compared against those observed in RNA under intracellular conditions.

Probing stress-induced effects on RNA and posttranscriptional modifications by LC-MS

254th National Meeting (2017)

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