Endogenous exposome is any exposure from the internal environment within the body which can have the propensity for developing various diseases throughout lifetime. It includes metabolism of byproducts from mitochondria, infection, inflammation, oxidative stress from reactive oxygen species, and so on. Oxidative stress can have an impact on DNA, resulting in the strand breakage by the abstraction of hydrogen at various carbon centers in deoxyribose moiety. Among the 5 carbons in this moiety, the hydrogen at the 5' carbon is more susceptible to abstraction compared to others. Nonetheless, the exact mechanism to understand how these are degraded are yet to be investigated. Here we present the investigation of oxidative damage products resulting from 5'-hydrogen abstraction. The goal of this project is to prove whether the degradation of the nucleoside modified at C5' position can be used as potential biomarkers to monitor oxidative stress. Such task can be initiated by identifying the degradation products derived from C5' oxidation of DNA. This involves the multi-step synthesis of C5' modified nucleoside followed by incubation studies using analytical techniques such as HPLC. Similar studies will be conducted in oligonucleotides.

Degradation from C5' oxidation and its adducts as potential biomarkers

254th National Meeting (2017)

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