Measuring sample throughput and purity of the collected compound are two parameters considered when measuring High Performance Preparative Chromatography efficiency. Part of achieving high efficiency is to maximize the sample loading. This is achieved by loading the sample so that the peak containing the desired compound barely touches the nearest impurity peak. An algorithm can be used to calculate maximum peak loading on a preparative column from an analytical HPLC test run. Using the peak widths and resolution from this injection, a maximum injection volume is calculated. This calculation saves time by reducing the number of test injections to optimize loading.

Prep LC: How much can I load?

255th National Meeting (2018)

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