Although there are several methods for producing enzyme loaded small unilamellar vesicles, there is significant need to develop scalable methods to produce large quantities of giant unilamellar liposomes that are loaded with functional enzymes for producing artificial cells and cell-like chemical reactors. In this poster we report our work for loading functional enzymes in solvent-free giant liposomes produced through the Paper-Abetted liPid hYdRation in aqUeous Solutions (PAPYRUS) method. Liposomes were grown at room temperature or higher, after which the proteins were loaded at 4 °C. The low temperature during the loading process and subsequent encapsulation retards the activity of endogenous proteases and reduces the denaturation of enzymes prior to experiments. The newly reported process of sequential growth and loading allows the formation of liposomes with membranes of complex compositions, including raft-forming compositions. The work thus expands the retinue of methods available for producing complex artificial cells.<br/>

Loading of functional enzymes into giant liposomes using cellulose paper

255th National Meeting (2018)

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