We report the development of a module for dual-output, dual-wavelength femtosecond lasers that facilitates multiphoton imaging and spectroscopy experiments and enables hyperspectral imaging with spectral resolution of up to 5 cm-1, comparable with spectral resolution of imaging using commercial picosecond laser sources. High spectral resolution is achieved by employing spectral focusing, using grating pairs to control the chirps in each laser beam. In contrast with fixed-length glass rods, grating pairs allow matching the spectral resolution and the linewidths of the Raman lines of interest. To demonstrate the performance of the module, we report the results of spectral focusing CARS and SRS microscopy experiments in various test samples. We also discuss the feasibility of using the developed module for variety of multimodal imaging and spectroscopy applications, such as single- and multi-color two-photon fluorescence, second harmonic generation, third harmonic generation, pump-probe, transient absorption, and others.

Spectral focusing multiphoton hyperspectral imaging with 5 cm-1 spectral resolution

256th National Meeting (2018)

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