Lignocellulosic biomass as renewable resources for biofuel and bioproduction.  Lignocellulosic biomass consists of three different polymers: cellulose, hemicellulose, and lignin.  To produce sugar monomers for the production, chemical pretreatment and enzymatic hydrolysis (saccharification) are required to deconstruct these polymers.  Commercial enzymes are expensive, inefficient, not reusable, and require different hydrolysis conditions.  <br/>This study explored one-pot enzymatic hydrolysis method to overcome the high cost and limitation during saccharification.  This research reports that saccharifying enzymes (cellullase and xylanase) can be produced in quantity from <i>E. col</i>.  These produced enzymes were capable of deconstruct cellulose and hemicellulose through one single step (one-pot) as these enzymes have similar optimal pH and temperature.  The crude cellulase showed 0.71 U/mL activities, equivalent to the activity of 3 g of commercial cellulase.  The crude xylanase showed 0.69 U/mL, equivalent to the activity of 0.28 g of commercial xylanase.  Reusability of crude enzymes was explored through development of cross-linked enzyme aggregates (CLEAs)/ magnetic-CLEAs (M-CLEAs).  We observed that such M-CLEAs would allow for reuse at least 3 times, suggesting that our one-step enzymatic hydrolysis approach is effective and can be a promising method to reduce overall cost of biofuel production.

One-step enzymatic treatment of lignocellulosic biomass using immobilized cellulase and xylanase

256th National Meeting (2018)

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