Noble metallic nanoparticles, such as silver nanoparticles (Ag-NPs) have been widely studied during the last years, mainly due to their excellent antibacterial properties, beside their potential applications in anticancer treatments. In this work, we report a general procedure to prepare highly reproducible and eco-friendly synthesis of Ag-NPs using potato starch as reducing and stabilizing agent, and water as the solvent. Furthermore, the potential anticancer properties of the synthesized nanostructure was evaluated on MDA-MB and SKBR3, i.e., breast cancer cell lines. The optical properties of the nanomaterials were characterized by UV-visible (wavelength of the maximum absorbance <sub>max</sub> = 405 ± 3 nm for Ag-NPs), whereas the stability of the colloids was analyzed by measuring their Zeta potential, parallel to these studies the colloids showed a stability in dispersion from more than 140 days. The structural properties were determined by X-ray diffraction (XRD), which confirms that the nanostructure possess a Face-Centered Cubic (fcc) crystal structure. The shape and morphology of the nanocrystal were studied by high-resolution transmission electron microscopy (HRTEM) and dynamic light scattering (DLS). The morphology of the nanoparticles were mainly spherical, whereas the size of the nanoparticles were 15 ± 2 nm for Ag-NPs. The yellowish silver colloids showed a cell viability around 75 to 85% on peripheral blood mononuclear cell (PBMC) after 24 h exposure determined by MTT assay using [AgNPs] = 25200 μM. The evaluation of the cytotoxic of the AgNPs colloids on the breast cancer cell lines, determined also by MTT assay, showed a cell death induction of approximately 80% and up to 90% on MDA-MB and SKBR3, respectively, after 24 h treatment with metallic nanoparticles. Finally, we plan as future study, the green synthesis of gold and bimetallic Ag/Au colloidal nanostructures for potential nanomedicine applications.

Highly reproducible and eco-friendly synthesis and characterization of silver nanocrystals and their potential anticancer therapeutic properties

256th National Meeting (2018)

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