The robust quantification and confirmation of known agrochemical metabolites from radiolabelled animal, plant and e-fate studies is an ongoing challenge in new product development. The challenge is often exacerbated by the trend in lower application rates, coupled with complex matrices. Furthermore, concentration of sample extracts can lead to degradation of unstable metabolites, which in turn result in fundamental changes in the quantification of the final radio profile.  In addition to techniques capable of positive structure identification (<i>e.g.,</i> MS and NMR), the OECD guidelines permit the application of co-chromatography of the metabolite(s) with known standards using “two dissimilar systems”. Whilst the use of truly orthogonal radio-HPLC methods and/or LC-MS is often regarded as the primary tools in industry for these purposes, far less merit is afforded to the application of radio-TLC, which is often seen as an awkward, ageing or even obsolete technique.  This presentation will highlight advantages of combining radio-HPLC methods and radio-TLC assays for the quantification and confirmation of known agrochemical metabolites. The application of these technologies will be underpinned by real world examples which will show that older techniques, such as TLC, can still have utility in the age of million dollar analytical instrumentation.

Using radio-HPLC and radio-TLC in tandem for the quantification and confirmation of known metabolites in support of agrochemical product development

256th National Meeting (2018)

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