We have developed a novel immunoPCR-based diagnostic platform which uses <i>in-house</i> self-assembled, ultra-detectable DNA-Avidin nanoparticles coated with poly(ethylene glycol) (PEG) that links the DNA amplification signal to the amount of target present in the sample. The non-exposed PCRable DNA labels coupled to the PEG coating reduces charge and non-specific “stickiness” enhancing assay sensitivity. We further optimized the detectability of the nanoparticles by designing a DNA reporter with four repeats of a unique synthetic DNA PCR target condensed in the nanoparticle. Using human chorionic gonadotropin hormone (hCG) as a model analyte our platform was able to quantitate the target hCG protein in picomolar concentrations using only standard lab equipment. Thus, our engineered nanoparticle-based iPCR technology can precisely quantitate protein biomarkers at ultra-low levels.

Immuno-nanoparticle PCR for ultrasensitive detection of proteins

2019 Spring National Meeting

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