Gout is a common inflammatory arthritis disease caused by inefficient metabolism of purines and the buildup of monosodium urate crystals in joints. Current maintenance therapies include the use of anti-inflammatory drugs, corticosteroids, and xanthine oxidase inhibitors with side effects. Metal-Assisted and Microwave-Accelerated Decrystallization (MAMAD) technique have been used as an efficient technique to decrystallize organic and inorganic crystals with gold nanoparticles (Au NPs) which act as “nanobullets when exposed to microwaves heating. In this study, the MAMAD technique was examined as a possible treatment aid for gout and used for the decrystalization of laboratory synthesized monosodium urate monohydrate based pseudo-tophi submerged in 20 nm Au NPs, encased in pseudo-bursa in three synthetic human joint models. Model 1 and model 2 were exposed (model 1: Closed joint, dehydration of pseudo-tophi after each MAMAD session and model 2: Open and closed joint, dehydration of pseudo-tophi after 7 MAMAD sessions.) to 7 MAMAD sessions (i.e., a session = 120 seconds at 5 W),) and model 3 exposed to 18 MAMAD sessions in a rotated closed synthetic joint, dehydration after every 3 MAMAD sessions (i.e., MWH on the right, top, and left sides). Percent mass reduction (PMR; i.e., decrystallization) evaluated after exposure to the MAMAD technique. Our results showed that the application of the MAMAD technique in model 1 pseudo-tophi had an average PMR of 8.30±0.56% (closed joint), model 2, pseudo-tophi had an average PMR of 11.5±1.82 % (closed joint)  and 5.89±1.59 %(open joint) and model 3 (rotated closed joint) 8.00 ± 2.53%. Our results demonstrate how the MAMAD technique can be a potential alternative treatment for gout with further studies.<br/>

Metal-assisted and microwave-accelerated decrystallization of pseudo-tophaceous aggregates in human joint models

2019 Spring National Meeting

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