Our studies of genome organization have led us in a number of directions, including super-resolution imaging of the genome. Here, we have developed an approach that enables the tracing of megabases of the human genome at super-resolution using sequence-specific fluorescent <i>in situ</i>hybridization Oligopaint probes and single-molecule localization microscopy (OligoSTORM and OligoDNA-PAINT). In brief, we have observed the 3D organization of chromosomal regions contributing to loops, contact domains, and compartments and, using homolog-specific Oligopaint (HOP) probes, have further observed differential packaging of maternal and paternal chromosomal regions. Then, through integrative modeling of genomic regions (IMGR), we have combined our images with Hi-C contact data to generate 3D models of homologous chromosomal regions.

Looking at chromosomes

2019 Spring National Meeting

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