Biothiols, such as cysteine (Cys) and glutathione (GSH), play many crucial roles in biological system. In the present work, we report a novel strategy for rapid identification of thiols by a facile fluorescence sensor array composed of a series of PDA/PEI48-Cu2+ sensors (PDA/PEI48-Cu2+4, PDA/PEI48-Cu2+4.5 and PDA/PEI48-Cu2+5) in acetate buffer with different pH. The sensing mechanism is based on that Cu2+ has strong affinity toward thiols, which prevents Cu2+ from binding PDA/PEIn, and the fluorescence property of PDA/PEIn was recovered to a certain degree. Distinct fluorescence response patterns were generated due to different thiols exhibited different affinities toward Cu2+. By using PCA, we realized the pattern discrimination of 8 thiols. Moreover, the proposed sensor array could discriminate the mixtures of thiols as well as chiral recognition of mixtures of Cys enantiomers, promising its potential practical usage. Significantly, the resultant practical application in real samples showed that it could be a fascinating assay for the development of non-invasive diagnosis.

Bioinspired Copolymers-Cu2+ hybrid sensor array for fluorescent pattern discrimination of thiols and chiral recognition of cysteine enantiomers in biofluids

2019 Spring National Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.