Nanopore sensors, which originate from ion channel proteins, are capable of sequencing genomic DNAs in single molecule. However, nanopore sensors have long been restricted to natural ion channel proteins or fabricated solid state apertures, which have limited spatial resolution for identifying a single nucleotide without any interference from its neighbour bases. Our recent investigation unveils an innovative approach to precisely engineer the restriction of biological nanopore with inorganic sensing adaptors for enhanced performances. Differently engineered nanopore sensors show highly specific recognition to a wide variety of molecules such as DNA bases, DNA base modifications and amino acids, which is inspiring for single molecule sequencing of DNA and protein with negligible errors. By digitally encoding fluorescence from Ca<sup>2+ </sup>ionic fluxes through each individual nanopore assisted by the Ca<sup>2+</sup> indicator dye Fluo-8, an array of such nanopore sensors (&gt;10^4) could also be visually monitored optically for enhanced sensing throughput.

Visually probing restriction engineered biological nanopores in a high-throughput nanopore array

2019 Spring National Meeting

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