Diets high in vegetables from the <i>Brassica</i> family can confer a variety of beneficial anticancer effects, largely due to the isothiocyanates (ITCs) produced via the enzymatic hydrolysis of glucosinolate natural products by myrosinase.  Prior studies have described novel methods to quantify and evaluate the enzymatic hydrolysis of natural and non-natural glucosinolates to ITCs.  This work sought to evaluate the conversion of benzyl glucosinolate and 2,2-diphenylethyl glucosinolate to their respective ITCs in a cellular setting, using an anticancer endpoint to measure the effectiveness of ITC formation.  In this study, the myrosinase-dependent antiproliferative effects of glucosinolates against human MCF-7 breast cancer cells were evaluated using a commercial MTS assay. When compared to the antiproliferative effects of pure ITCs, the results suggest that the conversion of glucosinolate to ITC occurs efficiently in a cellular setting, with reaction kinetics that are consistent with prior <i>in vitro</i> data.

Evaluation of the antiproliferation effects of glucosinolates against human MCF-7 cells

2019 Spring National Meeting

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