<br/>The biosynthetic pathways of NRPS-based siderophores are readily amenable for genome mining to predict the likely occurrence of new siderophores. NRPS-independent siderophore (NIS) synthetases have also been used to predict the occurrence of non peptidic siderophores, although relatively fewer of these types of siderophores have been predicted, in part because the biosynthetic clusters are much more variable than NRPS-based biosynthetic pathways, and also because the structures of NIS siderophores vary widely. We have been interested in the biosynthesis of citrate comprising siderophores formed by NIS synthetases. Many citrate siderophores, including aerobactin, ochrobactin C, petrobactin, etc are symmetrical with the same side arm modification on each terminal citrate carboxylate (Figure). Asymmetrically modified citrate yields a siderophore with at least one chiral center (i.e., the central carbon of citrate). We are investigating the occurrence, structure, biosynthesis, Fe(III) coordination and reactivity of these types of citrate-derived siderophores.

Genome mining for citrate-derived siderophores

2019 Spring National Meeting

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