Smart nanodevices that integrate the molecular recognition and signal production hold great promise for the point-of-care diagnosis (POC) applications. Here, we developed a nanodevice capable of sensing various bio-targets, and reporting signals on an easy-to-read platform. The nanodevice combines the mechanisms of dynamic DNA nanostructures (sensing unit) with the proximity assembly of enzyme/cofactor system (assembly unit). Our effort centers on methods to control the conformational switch of nanodevices for recognizing targets and to trigger the subsequent assembly of enzyme/cofactor structures for producing and amplifying signals. The nanodevices have been demonstrated to detect various targets of nucleic acids and small-molecule metabolites with colorimetric or fluorescence signals. The DNA-mediated proximity assembly circuit can be potentially applied to  engineer smart nanodevices for molecular diagnosis and be transferred to a paper-based POC tests.

DNA-mediated proximity assembly circuit for biochemical sensing

2019 Spring National Meeting

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