The improvements in instrument and column performance in liquid chromatography (LC) over the last 15-20 years have resulted in an almost 10-fold reduction in analysis time and 3-fold increase in separation efficiency. Nevertheless, the complexity of the samples emerging in life sciences (proteomics, metabolomics, lipidomics…), containing 10,000 or more analytes in a wide range of concentrations and physico-chemical properties (size, polarity, ionization state…) is so vast that it is impossible to even dream of ever achieving full resolution using unidimensional chromatography with the present state-of-the-art in instrumentation and columns. The rise of two-dimensional separation techniques, in combination with modern MS/MS systems, vastly increases the overall resolving power that can be achieved. Nevertheless, even 2D separations are ultimately limited by the efficiency of the chromatographic column in the individual dimensions.<br/><br/>In this talk, it will be attempted to look into the future of UHPLC, making a detailed assessment of current and future limitations of instruments (pressure, extra-column band broadening in injector, detector and connection tubing) and columns (elimination of viscous friction heat, flow distribution and frits at inlet and outlet). In addition, also potential improvements for particles will be discussed. These improvements can range from changes at the mesopore-level to changes of the particle shape using 3D-printing and silicon micromachining.

<b>Award Address</b> (ACS Award in Chromatography sponsored by MilliporeSigma). UHPLC: Where to go Next with Particles, Columns and Instruments?

2019 Spring National Meeting

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Award Address (ACS Award in Chromatography sponsored by MilliporeSigma). UHPLC: Where to go Next with Particles, Columns and Instruments?