There is considerable interest in the development of membrane mimetics for structural studies of membrane proteins. Recent studies have shown the benefits of detergent-free nanodiscs for functional reconstitution of membrane proteins. These nanodiscs are formed using membrane disrupting amphipathic helical proteins, peptides or polymers. Benefits of peptide and polymer based nanodiscs, the mechanism of lipid-nanodisc formation, influence of nanodiscs on amyloid aggregation and cellular toxicity, and functional reconstitution of membrane proteins for high-resolution NMR based structural studies will be presented. Synthesis, characterization, and nanodisc formation by a series of (styrene-based and also styrene-free) polymers that are tolerant to pH, divalent metal ions and temperature will also be discussed. Approaches to produce macro-nanodiscs and exploitation of their magnetic-alignment properties for solid-state NMR spectrosocpy will also be highlighted.

Polymer-based lipid nanodiscs

2019 Spring National Meeting

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