Stem cells are promising starting materials to derive functional cells and tissues for regenerative medicine. As such, there has been a need to monitor/validate stem cells in real-time, e.g., monitoring pluripotent stem cells prior to differentiation, validating the removed residual pluripotent stem cells after differentiation, for further clinical applications. This will reduce the risk of teratoma formation.1 Current detection techniques hinder real-time monitoring as they require labelling and are destructive. Instead, label-free techniques such as electrical-based detection (EBD) methods are promising for real-time measurements.2-3 Here, we observed a unique bioelectric signal of human embryonic stem cell using direct current-voltage measurements facilitated by few-layered 2D-MoS2 sheets.4 A 1.828 mA cell signal was achieved as well as multiple cell reading cycles demonstrating I ~ 1.9 mA. Native stem cell proliferation, viability and pluripotency were preserved. Molecular dynamics simulations elucidated the origin of the 2D-MoS2 sheet-assisted increase in current flow. This method promises a broadly applicable, fast and damage-free stem cell detection method capable of identifying pluripotency with virtually any complementary-metal-oxide-semiconductor circuits.

Two-dimensional materials that enhance human embryonic stem cell-signal detection

2019 Spring National Meeting

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