A technique was developed to easily create optimized both preparative HPLC and flash chromatography reverse phase methods from analytical HPLC/UHPLC runs. Preparative chromatography methods for normal phase chromatography are easily created from thin-layer chromatography (TLC) plates. Reverse phase methods are more difficult to create because TLC plates require a significantly longer time to run. Using HPLC/UHPLC for method development often requires complex scale-up calculations to determine gradient segment lengths to effect the same resolution.<br/> <br/>Time-on-Target uses a model compound to set a desired retention time on the preparative HPLC or flash chromatography system. The determined solvent composition from the preparative system is then used to calibrate the scouting gradient used on the analytical LC system. Compounds to be purified are run using the same scouting gradient as that used for the initial analytical calibration. Their retention time is adjusted by the calibrated scouting gradient to calculate a solvent composition which centers on an efficient focused gradient. The determined gradient is fast, saving solvent and reducing waste. Once calibrated, reverse-phase method development for reverse phase chromatography is faster than that using TLC for normal phase.<br/>

Time-on-target: Easy method development for reverse phase preparative chromatography

2019 Spring National Meeting

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