Affinity precipitation using elastin-like polypeptides (ELPs) conjugated to the Z domain of protein A has been previously demonstrated to be effective for the batch purification of mAbs with comparable performance to protein A chromatography. In this poster, we present an important new approach for the elution of mAbs from ELP-Z:mAb precipitates. Prior work necessitated several steps during the elution process, namely, resolubilization, elution and subsequent precipitation of ELP-Z. In the new approach which is being carried out in a continuous process, we can now elute the mAb directly from the precipitate, signifying simplifying the process. This proof of concept poster examines the potential of this approach and examines various operating parameters in order to optimize the performance. The impact of the nature of the precipitate on the kinetics of this elution process is also examined. Finally, we demonstrate that mAbs can be effectively eluted directly from the precipitates in a short time frame, thus facilitating the design of relatively simple continuous affinity precipitation processes.

Direct elution of purified monoclonal antibodies from ELP-Z-mAb precipitates

2019 Spring National Meeting

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