A technique was developed to easily create optimized chromatography methods for reversed phase flash chromatography from analytical HPLC/UHPLC runs. This has now been extended to normal phase preparative High Performance Chromatography.<br/>Time-on-Target uses a model compound to set a desired retention time on the chromatography system that is used to calibrate the scouting gradient used on the analytical LC system. Compounds to be purified are run using the same scouting gradient as that used for the initial analytical calibration. Their retention time is adjusted by the calibrated scouting gradient to calculate a solvent composition which centers an efficient focused gradient. The determined gradient is fast, saving solvent and reducing waste. After the initial calibration, method development for preparative chromatography is faster than that using TLC for normal phase.

Focused gradient generation: Easy method development for normal phase preparative chromatography

2019 Fall National Meeting

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