Disease-related biomarkers are normally expressed at ultralow levels during the early stage of diseases. We developed a series of biosensors based on micro-/nanoscale interfaces for the sensitive and selective detection of low-abundant biomolecules at single cell or single molecule level by utilizing spectroelectroanalysis and coupled techniques such as single molecular fluorescence. Reliable profiling of extracellular dopamine concentration in the central nervous system is essential for a deep understanding of its biological and pathological functions. Based on the specific recognition of boronate toward diol, and N-hydroxysuccinimide ester toward amine group, a simple and highly sensitive strategy was presented for dopamine detection by using surface-enhanced Raman scattering as a signal readout. Such dual-recognition-directed biosensing exhibited a high resistance to cerebral interference and was successfully applied to monitoring of dopamine in cerebrospinal fluid samples of patients. Membrane proteins are the main undertaker of many essential biological membrane functions. However, low abundance and the hydrophobicity of membrane proteins make their identification still a great challenge. A pragmatic and integrated protocol has been developed for large-scale characterization of hydrophobic membrane proteins, based on the surface functionalized mesoporous nanoreactors or microfluidic droplet reactors, acting as both extractors for hydrophobic substrate capture and nanoreactors for efficient in situ digestion.

Microscale interfaces for sensitive biosensing and comprehensive identification of biomolecules

2019 Fall National Meeting

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