Nanoparticle interaction with biological membranes is expected to be modulated by coatings of proteins acquired in biological fluids, as well as by peripheral and integral membrane proteins. This presentation will highlight our recent progress in understanding the roles of proteins in nanoparticle-membrane interactions. We find that the initial nanoparticle surface coating impacts the interactions with model cell membranes by determining the assemblage of complexing proteins, which in turn influences subsequent interaction with model biological membranes. Using dissipative quartz crystal microbalance, nanoplasmonic sensing, and molecular dynamics simulations, we find that the peripheral membrane protein cytochrome <i>c</i> mediates membrane interaction of anionic nanoparticles which would not otherwise interact with the membrane. Combining a mass spectrometry-basedfootprinting approach and computational molecular dynamics methods allowed the preferred nanoparticle-binding site on the protein to be ascertained. Taken together, our results highlight the importance of the surface chemistry of both the nanoparticle and the membrane in predicting nano-bio interactions.

Modulation of nanoparticle-membrane interaction by proteins

2019 Fall National Meeting

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