Characterization of the overall topology and inter-subunit contacts of protein complexes, and their assembly/disassembly and unfolding pathways, is critical because protein complexes regulate key biological processes, including processes important in understanding and controlling disease. Tools to address structural biology problems continue to improve.  Native mass spectrometry and associated technologies are becoming an increasingly important component of the structural biology toolbox.  When the mass spectrometry approach is used early or mid-course in a structural characterization project, it can provide answers quickly using small sample amounts and samples that are not fully purified.   Integration of sample preparation/purification with effective dissociation methods, ion mobility, and computational approaches provide a MS workflow that can be enabling in biochemical, synthetic biology, and systems biology approaches.  Beyond what MS can provide as a stand-alone tool, MS can also guide and/or be integrated with other structural biology approaches such as NMR, X-ray crystallography, and cryoEM.  MS can determine whether the complex of interest exists in a single or in multiple oligomeric states and can provide characterization of topology/intersubunit connectivity, and other structural features.  Examples will be presented to illustrate the role surface-induced dissociation (SID) can play in guiding a structural biology workflow and will include designed protein complexes and isolated or recombinant protein and nucleoprotein complexes.  Recent modifications of SID devices will be discussed. <br/>

Role of surface collisions in an MS-based structural biology approach

2019 Fall National Meeting

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