Mitochondrial electron transfer chain is consisted of enzyme complex I to V that are located in inner membrane of mitochondria. Simple enzymatic activity of as-isolated each complex has been measured so far, but steady state kinetics of each complex in mitochondria alive has not been studied in depth. In this study, steady state kinetic analysis for bovine heart mitochondrial complex I to V was performed using varying concentration of corresponding substrates. Binding affinity and catalytic activity was obtained by fitting the data to Michaelis Menten equation. This is the first attempt to get exact kinetic values that should provide profound information to evaluate mitochondria function practically in advance.

Steady-state kinetic analysis of the bovine heart mitochondrial enzymes

2019 Fall National Meeting

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