Chungkookjang with potent thrombolytic activity may have anti-stroke activity. We hypothesized that chungkookjang made with B. amyloliquefaciens SRCM100730(CKJ730) and SRCM100731(CKJ731) would protect against ischemic stroke and post-stroke hyperglycemia. The hypothesis was evaluated in Mongolian gerbils with transient occlusion of the carotid arteries, and its mechanism was explored. Gerbil had 4.3% dextrin(control and normal-control), CSB, CKJ730, or CKJ731 in 40 energy% fat diets for 3 weeks after which gerbils, except normal-controls, had artery occlusion for 8 min and continued taking the assigned diet. CKJ730 and CKJ731 supplemented diets had thrombolytic activity and prevented neuronal cell death the most. Consequently, they improved short-term memory and spontaneous alteration compared to the control. Neurological symptoms including drooped eyes, crouched posture, flexor reflex, walking patterns and active time improved the most in CKJ730 and CKJ731 over time. The force to grip the grid was also increased in CKJ730 and CKJ731 the most. Blood flow measured by Doppler was increased in CKJ730 and CKJ731 compared to the control. Post-stroke glucose metabolism, as measured by OGTT and IPITT, was deteriorated in the control more than the normal-control and CKJ730 improved glucose metabolism better than the normal-control, and was associated recovery of β-cell mass which was decreased by β-cell apoptosis due to artery occlusion. Along with the disturbance of glucose metabolism, serum TNF-α and IL-1β levels were lower in CKJ730 and CKJ731 than the control. CSB also improved glucose metabolism and decreased inflammatory cytokines but its effects were smaller than CKJ730 and CKJ731. Clostridia increased and Bacteriodia slightly decreased in the control group, compared to the normal-control. CKJ730 and CKJ731 changed the amounts of Bacteriodia and Clostridia as similar to normal control. In conclusion, CKJ730 and CKJ731 may be useful as a preventive measure for ischemic stroke by improving blood flow, inflammation, and gut microbiome.

Chungkookjang, a fermented soybean food, fermented with Bacillus amyloliquefaciens SRCM100730 and SRCM100731 protected against ischemic stroke and post-hyperglycemia by improving blood flow in gerbils

2019 Fall National Meeting

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