Dry edible beans are an excellent source of dietary proteins and peptides. Great Northern (GN) beans are a market class of dry edible bean and a valuable agricultural commodity of Nebraska. These GN beans are generally cultivated in the western part of the province, where annual precipitation rates are meager. So different bean varieties were developed to grow in these environmental conditions. However, it is not known how the different growing conditions may modulate the biological activity of the bean-derived proteins and peptides. Therefore, the primary objective of the present study is to evaluate the effect of growing conditions on protein digestibility and the anti-oxidant activity of the whole bean hydrolysate. GN beans grown in both drought and irrigated conditions were first soaked overnight and cooked at 95oC for 90 minutes. Alcalase then used to hydrolyze the cooked GN beans at pH 8.5 and temp 60oC for 3 hrs. The degree of hydrolysis (DH) was measured by the pH-stat method, and the antioxidant capacity of the cooked whole bean hydrolysate was measured by the ABTS (2,2’-azino-bis-3ethylbenzothiazoline-6 sulphonic acid) radical scavenging assay. The beans grown in irrigated conditions have a significantly high amount of total protein (22.8%) compared to the beans grown in drought conditions (18.9%). However, the drought-beans have exhibited a slightly higher degree of hydrolysis (DH: 19.2% ± 1.9), not statistically significant, compared with irrigated-beans (DH: 16.9% ± 1.2). Furthermore, the ABTS radical scavenging assay resulted in an IC50 value of 0.8 mg/mL for the drought-bean-hydrolysate and 0.7 mg/mL for the irrigated-bean-hydrolysate. Thus, the results obtained from the study so far indicates that the GN beans grown in drought conditions despite having a low amount of protein had exhibited similar Alcalase digestibility and antioxidant activity as compared to the beans grown in irrigated conditions. Thus, this study concludes that the beans grown in different environmental conditions do not affect the biological activity of bean-derived peptides.

Effect of growing conditions on the digestibility and anti-oxidant activity of the Nebraskan Great Northern dry edible bean (Phaseolus vulgaris)

2019 Fall National Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.