Self-assembly of proteins into higher order super-structures is ubiquitous in biological systems. Computational and rational design strategies are powerful methods for design of synthetic protein complexes with high accuracy but reorted protein complexes lack dynamic behavior. On the contrary, protein engineering using chemical strategies offers excellent possibilities for the design of protein complexes with <b>stimuli-responsive functions and adaptive behavior</b>. However, designs based on chemical strategies are not accurate and therefore yield polydisperse samples. Here, Micelle-assisted activity-based protein labeling technology has been developed to synthesize library of facially amphiphilic synthetic proteins, which self-assemble to make protein complexes through hydrophobic interaction. The broad utility of this method is demonstrated by design of most <b>sophisticated stimuli-responsive protein complex</b> that can be programmed to assemble/disassemble in a reversible/irreversible fashion using pH or light as a trigger.

Rational design of supramolecular dynamic protein assemblies using a micelle-assisted activity-based protein labeling technology

2019 Fall National Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.