A denatured bubble-mediated chain exchange-induced isothermal nucleic acid detection technique (SEA) was developed to detect Vibrio parahaemolyticus in seafood. This method is based on the respiration of DNA's own denatured bubbles, which makes the DNA double strands dynamically dissociated. The reaction requires only one DNA polymerase to participate in the reaction. It can detect 1.0×10-13 M, and has strong specificity and anti-interference ability. Compared with other traditional methods, the reaction can be detected within 20-40 min. The SEA method can directly detect the bacterial liquid of Vibrio parahaemolyticus, avoiding the cumbersome steps of extracting genomic DNA and the technical requirements of the experimental personnel, and greatly shortening the time of experimental detection. This method may play a role in immediate detection, animal disease surveillance, and environmental monitoring.

Detection of <i> Vibrio parahaemolyticus </i> in marine foods based on denatured bubble-mediated isothermal nucleic acid amplification

2019 Fall National Meeting

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Detection of Vibrio parahaemolyticus in marine foods based on denatured bubble-mediated isothermal nucleic acid amplification