The use of biodegradable materials as drug delivery devices have recently gained a lot of interest in the treatment of bone infection because of their biodegradability, biocompatibility, ease of modification, etc. This study describes the fabrication of a biodegradable nanocomposite having one-dimensional fibrous structure from bio-ceramic material nano-hydroxyapatite (nHAp) and biopolymer Poly Lactic Acid (PLA) using electrospinning technique. nHAp was produced from locally produced biowaste eggshells using the wet chemical method. The average particle size of nHAp was obtained at about 25~35 nm by Transmission Electron Microscopic Analysis (TEM) and was found to be highly crystalline by X-ray Diffraction (XRD). Fourier transform infrared spectrophotometer (FTIR) and X-ray Diffraction (XRD) showed molecular interaction and chemical linkages between nHAp and PLA. Degradation study of the prepared PLA-nHAp nanocomposite was carried out for 30 days both in deionized water and phosphate buffer solution (PBS) of pH 7.4 where degradation occurred faster in PBS than water. Cytotoxicity analysis of both Vero cells and BHK-21 cells showed that the nanocomposite is non-toxic and biocompatible. Gentamicin was loaded into the nanocomposite via in-situ process to investigate the in-vitro drug delivery system of the PLA-nHAp nanocomposite. An initial burst release was observed after 1 to 2 weeks in phosphate buffer solution at different pH. After burst release, the nanocomposite showed sustained release behavior over the remaining weeks with a release of 80% at pH 5.5. Overall, the studies confirmed that the prepared PLA-nHAp electrospun nanocomposite can be a potential antibacterial drug carrier for controlled drug release applications specially in bone diseases.

Biodegradable polylactic acid (PLA)/nano-hydroxyapatite (nHAp) electrospun nanocomposite for sustained drug delivery

ACS Spring Meeting 2021

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.