Epigenetic marks such as the acetylation of histones change substantially with aging or UV exposure. Increase of histone acetylation could be related to the decrease of SIRT-1 deacetylase enzyme and its NAD+ co-factor produced by mitochondria at the same time as ATP. SIRT-1 appears as a highly relevant epigenetic marker of cell activity while ATP and mitochondrial activity materialize the measurable consequences. The purpose of this study was to evaluate the capacity of an algae extract to reactivate SIRT-1 synthesis, mitochondrial activity and ATP production, to improve cell vitality thus limiting senescence and the cell aging signs. Experimentation were performed on normal human dermal fibroblasts (NHDF). Capillary electrophoresis-based protein analysis system (Sally Sue) was used to quantify SIRT-1 protein. ATP production was evaluated with luminescent assay and mitochondrial activity with spectrophotometry. NHDF were treated for 24h by 0.002% of resveratrol, a known inducer of SIRT-1, 1% of SVF as inducer of mitochondrial activity and ATP and 0.02% of a Fucus vesiculosus extract enriched in fucoidans. Resveratrol significantly stimulated by 35% the synthesis of the SIRT-1 protein versus the untreated control. Interestingly, 0.02% of the Fucus vesiculosus extract induced in a better extent the synthesis of SIRT-1 protein (+ 42%). Moreover, the Fucus vesiculosus extract at 0.02% significantly stimulated by respectively 138% and 258% the mitochondrial activity and ATP production versus the untreated control. SIRT-1 is an epigenetic regulator and protector of cell longevity through histone deacetylation and is associated with cell energy and mitochondrial activity. Our specific Fucus vesiculosus extract has particularly showed, its ability to stimulate SIRT-1 as efficiently as resveratrol, but without stability issue in formulation. Altogether, these results demonstrated that this Fucus vesiculosus extract may act through an epigenetic process to increase cell vitality into the dermis, and to improve cell aging signs such as skin roughness and dullness.

ESDR178 - Activation of epigenetic Sirt1 synthesis by a fucus vesiculosus extract

50th Annual Meeting of the European Society for Dermatological Research. VIRTUAL MEETING