Rosacea, a chronic skin disorder, is characterized by inflammation and vascular abnormalities of the central facial skin. Cathelicidin LL-37 and VEGF are both specific contributors to physiopathology of Rosaceae and involved in inflammatory and angiogenesis pathways respectively. The aim of this work was to assess the soothing and anti-redness properties of a dermo-cosmetic ingredient in several models exposed to the different components of the rosacea environment. Firstly, the use of human normal keratinocyte primary cell line (NHEK) after a tangerine extract pre-treatment allowed us to show a decrease of VEGF and MMP9 gene expression and IL8 cytokine release after an stimulation by Rosaceae-like cocktail (TLR agonist, LL37, TNFα). In the same way, after a stimulation with Substance P on this previous cell line, an inhibition of the production of cytokines IL-1β and TNFα could be demonstrated. Then, the potency on angiogenesis axis of this compound could be confirmed on a cocultured NHDF/HMVEC based VEGF stimulated Pseudotubes model as well as on the Ca2+ production of a AoSMC model. Moreover, this compound once formulated in a dermo-cosmetic cream was able to inhibit the production of chemokines involved in neurogenic inflammation and EGFR1 pathways in a rosacea reconstructed human epidermis model. These data suggest that a topical Dermo-Cosmetic product with this tangerine extract could be useful to protect against Rosaceae burden in targeting inflammatory and vascular scales.

ESDR242 - High potency activities of a Tangerine extract to target Rosaceae imprints

50th Annual Meeting of the European Society for Dermatological Research. VIRTUAL MEETING