Mesenchymal stem cell-based therapies are emerging as promising approach to promote cutaneous regeneration of chronic wounds. We assessed here the regenerative potential of a patch concentrating mesenchymal stem cells extracted from the human fat, i.e. adipose-derived stem cells (ASC), in a gelatin matrix. ASC cultures were derived from the subcutaneous abdominal fat of four patients. Principal component analysis on microarray data revealed that ASC grown within the patch upregulate the transcription of genes important for wound healing, including VEGF, CXCL12and FGF7. Secretome analysis confirmed the observed upregulations at the protein level. ASC-patches subcutaneously transplanted in immunodeficient Nu/Nu nude were early invaded by new vessels. Of note, transplanted mice did not form any tumors. Marked vasculogenesis was similarly demonstrated in a preclinical chick chorioallantoic membrane model when compared to empty matrix or with a matrix containing human fibroblasts. Finally, ASC-patches prepared from inguinal fat pads of Wistar rats were used to treat full-thickness skin wounds created on the foot of syngeneic rats whose paw was rendered ischemic by surgical resection of the femoral artery. The ASC-patch allowed a faster wound closure when compared to an empty matrix. Together, we provide here evidence that an ASC-containing matrix represents a promising approach for the treatment of chronic wounds thanks to its healing and vasculogenic properties.

ESDR369 - An Adipose-derived Stem Cell-engineered matrix represents a promising treatment for chronic wounds.

50th Annual Meeting of the European Society for Dermatological Research. VIRTUAL MEETING