This study describes the quantification of the inflammation parameters cytokines and eicosanoids in dermal interstitial fluid (dISF) samples during the process of wound healing. The dISF samples were collected with the minimally invasive sampling method open flow microperfusion (OFM) and split skin wounds were induced in farm pigs at a depth of 0.6 mm. The cytokine and eicosanoid concentrations were monitored in six and two days old wounds and compared to fresh wounds (directly after wounding) and to control sites (non-wounded skin). Two OFM probes were inserted in the wound edges and two OFM probes on the wound ground of each wound. Diluted dISF samples were collected with OFM over a period of six hours in two sequential sampling intervals, each lasting for three hours. For the measurement of the porcine cytokines a multiplexing platform using the MSD system was established, and the eicosanoids were quantified using liquid chromatography mass spectrometry. We successfully assessed the levels of commonly monitored cytokines together with eicosanoids during the wound healing process. Cytokine as well as eicosanoid concentrations in the wound ground were higher in two and six day old wounds than in fresh wounds or in the control sites. The measured cytokine and eicosanoid concentrations were lower in samples taken from wound edges than those taken from wound grounds. Furthermore, the cytokine and eicosanoid concentrations found in samples collected in the first OFM sampling interval were higher than those found in samples collected in the second interval. The obtained results showed that the combination of tissue-specific sampling using OFM and quantitative measurements of the inflammatory markers cytokines and eicosanoids creates new avenues for a better understanding of the process of wound healing.

ESDR371 - Quantitative profiling of cytokines and eicosanoids in diluted interstitial fluid samples from a pig model for wound healing

50th Annual Meeting of the European Society for Dermatological Research. VIRTUAL MEETING