INTRODUCTION: An antioxidant can be defined as a molecule capable to prevent or slow down the oxidation of other molecules, biological substrates such as lipids, proteins or nucleic acids. Secondary metabolites are non-essential natural products for the organism that produces, but provide an advantage to their survival, thus presenting therapeutical activities.
PURPOSE: To measure antioxidant activity of hydrophobic metabolites ofmarine Gordonia sp. with the tachnique ofDPPH-HPLC.
MATERIALS AND METHODS: Growth ofmarine Gordonia sp. An extraction with hexane(Ext 1) followed by isopropylic eter(Ext 2) was carried out. Extracts were characterized with metabolicscreening of extracts with chemical tests suchas Brayme’stest, foamtest, Salkowski’stest, among others; followed bypreliminar characterization withTLC usingas mobilepase heaxne:ethyl acetate(95:5) and HPLC in a Waters Symmetry(C18) column with acetonitrile:wáterat 210 nm. UV-Vis spectra was obtained from200 to 800 nm. After theTLC was developed, a solution ofDPPH was sprayedin to theplaque and incubatedat 36°C for 30 minutes, yellow points were observed for compounds with antioxidant activity. Antioxidant activity measuremente withDPPH-HPLC was carried out by mixing the extract (0.5-8 mg/mL) withDPPH solution and was agitated, followed by incubation of samplesat 36°C for30 minutes. HPLC conditions were the same as HPLC análisis of extracts at 517 nm forDPPH detection.
RESULTS AND DISCUSSION: Presence of tannins, flavonoids, terpenoids, steroids, coumarines, alkaloids and proteins were found with the metabolic screening.With DPPH-TLC three compounds with antioxidant activity were found on Ext 1, while in Ext 2 one single compound presented antioxidant activity. Chromatograms of both extracts can be seen where in Ext 1 a compound at 6.013 minutes similar to lysine (0.900 minutes) was observed, while Ext 2 presented a metabolite at 36.393 minutes, which was similar to naringin (31.666 minutes). Thus, concluding that these metabolites are hydrophobic analogues of the compared standards. With DPPH-HPLC it was determined that Ext 2 presented a better antioxidant activity (maximum activity of 89.12%) than Ext 1 (maximum activity of 21.97%)
CONCLUSIONS: Ext 2 presented the best antioxidant activity with DPPH-HPLC due to the compound present at 39.393 minutes.


**INTRODUCTION:** An antioxidant can be defined as a molecule capable to prevent or slow down the oxidation of other molecules, biological substrates such as lipids, proteins or nucleic acids. Secondary metabolites are non-essential natural products for the organism that produces, but provide an advantage to their survival, thus presenting therapeutical activities.
**PURPOSE:** To measure antioxidant activity of hydrophobic metabolites ofmarine _Gordonia_ sp. with the tachnique ofDPPH-HPLC.
**MATERIALS AND METHODS:** Growth ofmarine _Gordonia_ sp. An extraction with hexane(Ext 1) followed by isopropylic eter(Ext 2) was carried out. Extracts were characterized with metabolicscreening of extracts with chemical tests suchas Brayme’stest, foamtest, Salkowski’stest, among others; followed bypreliminar characterization withTLC usingas mobilepase heaxne:ethyl acetate(95:5) and HPLC in a Waters Symmetry(C18) column with acetonitrile:wáterat 210 nm. UV-Vis spectra was obtained from200 to 800 nm. After theTLC was developed, a solution ofDPPH was sprayedin to theplaque and incubatedat 36°C for 30 minutes, yellow points were observed for compounds with antioxidant activity. Antioxidant activity measuremente withDPPH-HPLC was carried out by mixing the extract (0.5-8 mg/mL) withDPPH solution and was agitated, followed by incubation of samplesat 36°C for30 minutes. HPLC conditions were the same as HPLC análisis of extracts at 517 nm forDPPH detection.
**RESULTS AND DISCUSSION**: Presence of tannins, flavonoids, terpenoids, steroids, coumarines, alkaloids and proteins were found with the metabolic screening**.**With DPPH-TLC three compounds with antioxidant activity were found on Ext 1, while in Ext 2 one single compound presented antioxidant activity**.** Chromatograms of both extracts can be seen where in Ext 1 a compound at 6.013 minutes similar to lysine (0.900 minutes) was observed, while Ext 2 presented a metabolite at 36.393 minutes, which was similar to naringin (31.666 minutes). Thus, concluding that these metabolites are hydrophobic analogues of the compared standards. With DPPH-HPLC it was determined that Ext 2 presented a better antioxidant activity (maximum activity of 89.12%) than Ext 1 (maximum activity of 21.97%)
**CONCLUSIONS:** Ext 2 presented the best antioxidant activity with DPPH-HPLC due to the compound present at 39.393 minutes.


Antioxidant activity of hydrophobic metabolites from Gordonia sp. by DPPH-HPLC

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