The binding of RAD51 protein to single-stranded DNA is important to the process of homologous recombination (HR). Although HR is a very important DNA repair pathway, there remain gaps in our understanding about the physical and structural properties of this mechanism. Using Atomic Force Microscopy (AFM), the binding process was visualized giving greater insight about the process of HR. Before imaging the DNA/protein complex, the DNA and protein were first imaged by themselves, and then combined to create the nucleoprotein complex to image. The collected images will help create a better understanding of the physical and structural properties of HR.

Visualizing human RAD51 protein binding to single-stranded DNA using atomic force microscopy

ACS Spring 2022

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