In central nervous system, neurotransmitters are responsible for transferring signals from pre-synaptic vesicles to post-synaptic receptors. The most abundant amino acid in brain, glutamate, is the primary excitatory neurotransmitter which plays a major role to regulates many functions in human body such as memory, learning, and long-term depression. Fluorescence detection as a noninvasive tool provides high spatial resolution in compared with other conventional methods. To this aim, various probes have been developed to monitor neurotransmitters in cells.<br/>In this work, we plan to develop a turn-on fluorescence sensor based on coumarin-3-aldehyde scaffold to selectively recognize gamma-amino acids neurotransmitters like glutamate and GABA in live cells. This neurosensor binds to the analyte through formation an iminium ion and a boronic esther. These two binding sites enable the sensor to selectively bind to amino acids rather than simple amines. Spectroscopic and binding data will be presented.

Development of selective fluorescence neurosensor for neurotransmitter monitoring in live cells

ACS Spring 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.