Late stage differentiation of dental pulp stem cells along can be accomplished in the absence of other common induction factors, such as dexamethosone, via surface preparations. Using a combination of complementary techniques, we show that genetic upregulation of late stage differentiation markers is only accomplished on surfaces where the matrix collagen secreted by the cells, self assembles into the natural banded structure, enabling templated mineral deposition. We show that self assembly of collagen can be triggered mostly by the surface chemistry, topography, or charge rather than the bulk composition of a material. Hence differention can be enabled on general classes of structures, as long as the specific chemical triggers for differentiaiton can be deposited in 3-D. This has important implications in the design of prosthetics using 3-D printing where the bulk materials are chosen for their mechanical or biodegradable properties, while surface coatings engineered for osseointegration and osteo or odonto differentiaiton can be applied separately using , electrospinning (1a,b), atomic layer deposition (1c,d), or energetically driven surface segregation.

Templated biomineralization and differentiation of dental pulp stem cells on printed structured surfaces

ACS Spring 2022

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