Methods to produce geometrically and physiologically complex 3D cell models have advanced over the past years thanks to 3D printing and an increased knowledge of the environment, including the importance of the extracellular matrix and metabolite production. This has allowed 3D in vitro models to be used as a stepping-stone, or even alternative, to in vivo studies. We are interested in producing controlled environments in which factors such as local pH, temperature, degree of cellular migration, and metabolite production can be studied. To do so we have devised a system in which both inorganic nanoparticles and organic matrixes can be combined, to provide sensing or stimulating triggers within a supportive structure, respectively. By careful tuning of the chemical composition of both components, 3D scaffolds with high resolution can be printed, providing a biocompatible support for cellular growth.<br/>Specifically, we are interested in applying gold nanoparticles (AuNPs) which allow Surface Enhanced Raman Spectroscopy (SERS) imaging. SERS allows us to follow cell migration and division in a non-disruptive manner, avoiding the commonly encountered photobleaching and phototoxicity that occurs with fluorescence microscopy. Furthermore, imaging depths are increased thanks to the use of NIR excitation sources.<br/>We will present various advances in this field which includes the use of these hybrid biocompatible scaffolds for cellular pH and temperature sensing, drug penetration, and tumor growth dynamics.

Combining SERS with 3D matrixes to better understand cellular behaviour

ACS Spring 2022

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