Bone tissue defects caused by disease, trauma, aging or genetic factors emerged as one of the main factors that endanger human health. At present, advanced development of bone tissue engineering and regenerative medicine focused on the biomaterials regulated stem cell for responsive differentiation. <i>In vivo</i> transplantation of allogeneic bone materials has the needs of both osteogenic and immune regulation function. In this study, we utilized the extensively proved biocompatible layered double hydroxide (LDH) nanoparticles as the nanocarrier of graphene quantum dots (GQD), the functional loading was validated by characteristics analysis of scanning electron microscopy (SEM), elemental mapping, surface zeta potential, X-ray diffraction (XRD) and Fourier transform infrared (FTIR). Further, we investigated the cellular uptake of nanoparticles in bone marrow derived mesenchymal stem cells (BMSCs), the significant enhanced endocytosis was occurred in LDH-GQD treated groups. The enhanced osteogenic differentiation abilities of LDH-GQD were systematically investigated through alkaline phosphatase staining, alizarin red staining and qPCR analysis. In addition, the anti-inflammatory regulation of LDH facilitated the phenotypic transition of macrophage in LDH- GQD nanocomposites. Overall, the successful construction and functional validation of nanomaterials in this study providing clinical therapeutic potential in bone defects regeneration.

Effects of graphene quantum dots loading with immunomodulatory layered double hydroxide nanoparticles for enhanced osteogenesis function

ACS Spring 2022

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