Studies have shown that the L protein of the single-stranded RNA phage MS2 causes lysis of Escherichia coli and that L interacts with DnaJ, a host chaperone protein, during the lysis process. But, whether DnaJ is absolutely necessary for L directed lysis to occur is not known. There remains the possibility that other proteins are involved in mediating L directed lysis. As a preliminary control experiment and to further explore the role of DnaJ in L directed cell lysis, we created inducible versions of MS2 L and E. coli DnaJ. We hypothesize that our recombinant proteins will interact directly in vitro. To provide results in support of our prediction, E. coli DnaJ will be cloned and expressed in combination with an epitope tagged MS2 L protein. Then two immunoprecipitations will be performed: HA-L with S-tagged DnaJ and HA-L with untagged DnaJ. The complete results for all the experiments will be presented.

Analysis of the MS2 L directed cell lysis mechanism in Escherichia coli

ACS Spring 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.