Objectives: The enzymatic debridement of burn wounds is a standard procedure for burn eschar removal. Upon selective enzymatic digestion of thermally injured tissue, the choice with regards to subsequent treatment, however, is still subject to debate. Middermal wounds in particular present a challenge as classic burn surgery approaches, i.e. excision an split thickness skin grafting, and more conservative approaches clash.
Regenerative medicine is an uprising innovative field in science and medicine that fosters autologous reparative functions. Various treatment strategies have evolved over the years which are primarily used in cosmetic and reconstructive surgery. Surprisingly, modern regenerative medicine techniques are rarely applied for burn wounds despite the fact that burn patients may benefit from little invasive treatment modalities significantly.
In the present study, the feasibility of regenerative medicine techniques including fat progenitor cells (stromal vascular fraction, SVF) and growth factor concentrates (platelet-rich fibrin, PRF) on enzymatically debrided wounds were investigated.
Methods: The study was conducted at the Burn Center of the University Hospital Zurich. The SVF of was isolated through a semi-automated system with lipoaspirates harvested from non-injured areas. Also, PRF was harvested from peripheral venous blood through standard kits. Both were mixed and applied on mid-dermal burn wounds 24-48 hours after enzymatic treatment with Nexobrid®. The outcome was analyzed retrospectively.
Results: A total of seven patients (4 male, 3 female; average age 47.71 years) were treated with the outlined technique and evaluated to date. Despite complete healing in four patients, three patients required additional tangential excision with subsequent split thickness skin grafts (STSGs). No long term risks or complications were observed.
Conclusion: In theory, Nexobrid® maintains a feasible matrix, similar to scaffolds used in tissue engineering approaches, for progenitor cells and regenerative factors. Despite excitement about the great potential of regenerative medicine, a clear understanding and right decision making is pivotal.

**Objectives:** The enzymatic debridement of burn wounds is a standard procedure for burn eschar removal. Upon selective enzymatic digestion of thermally injured tissue, the choice with regards to subsequent treatment, however, is still subject to debate. Middermal wounds in particular present a challenge as classic burn surgery approaches, _i.e._ excision an split thickness skin grafting, and more conservative approaches clash.
Regenerative medicine is an uprising innovative field in science and medicine that fosters autologous reparative functions. Various treatment strategies have evolved over the years which are primarily used in cosmetic and reconstructive surgery. Surprisingly, modern regenerative medicine techniques are rarely applied for burn wounds despite the fact that burn patients may benefit from little invasive treatment modalities significantly.
In the present study, the feasibility of regenerative medicine techniques including fat progenitor cells (stromal vascular fraction, SVF) and growth factor concentrates (platelet-rich fibrin, PRF) on enzymatically debrided wounds were investigated.
**Methods:** The study was conducted at the Burn Center of the University Hospital Zurich. The SVF of was isolated through a semi-automated system with lipoaspirates harvested from non-injured areas. Also, PRF was harvested from peripheral venous blood through standard kits. Both were mixed and applied on mid-dermal burn wounds 24-48 hours after enzymatic treatment with Nexobrid®. The outcome was analyzed retrospectively.
**Results:** A total of seven patients (4 male, 3 female; average age 47.71 years) were treated with the outlined technique and evaluated to date. Despite complete healing in four patients, three patients required additional tangential excision with subsequent split thickness skin grafts (STSGs). No long term risks or complications were observed.
**Conclusion:** In theory, Nexobrid® maintains a feasible matrix, similar to scaffolds used in tissue engineering approaches, for progenitor cells and regenerative factors. Despite excitement about the great potential of regenerative medicine, a clear understanding and right decision making is pivotal.

P.065 Regenerative Medicine Approaches after Enzymatic-Debridement

Dear friends and colleagues,

We will be pleased to welcome you in Turin (Italy) for the 19th European Burns Association´s Congress in 2022. Italy is honored to hold the meeting of the European Burns Association for the second time, after Verona in 1995. Turin is easily reachable from everywhere in Europe, either travelling by car, train or choosing flight connections. During the meeting, held in the renewed industrial center of the main Italian historical automotive factory (i.e. Lingotto-FIAT), we will meet international burn specialists, focusing on standards of care, progresses in burn treatment, strategies to face disaster situations, with a special attention to education of younger colleagues. However, in your spare time, you might enjoy the sweet days of Italian late summer, simply wandering in the green parks and gardens along the two rivers which cross the city, or you might decide to discover the numerous art galleries, churches, palaces, libraries, walking across elegant squares and large boulevards, passing through centuries of art and history; finally, you may choose to immerse yourself in some of the most exciting museum experiences, such as the Cinema Museum and the Turin’s Egyptian museum.

Looking forward to seeing you all in Turin for the 19th European Association´s Congress in 2022!
Maurizio Stella, Congress President, Turin Burn Centre