and 1 other(s)
Bone marrow derived mesenchymal stem cells (MSCs) exhibit the potential to undergo chondrogenesis in vitro, forming de novo tissues with a cartilage-like extracellular matrix that is rich in glycosaminoglycan and collagen type II. However, it is commonly accepted that MSCs comprise an inhomogeneous population of cells, and the fate of individual subpopulations during this differentiation process is not well understood. While changes in the transcriptomic profiles of MSC chondrogenesis had been profiled through bulk analyses of pellet samples, the impact of clonal architecture remains poorly understood. Moreover, strategies targeting the molecularly distinct subpopulations of that drive the clonal structure of MSC chondrogenesis are largely nascent. To address this problem, we have employed single-cell RNA sequencing (scRNA-seq) to dissect the clonal sub-structure of pellets derived from MSC chondrogenesis. As a proof of concept, we highlighted the potentials of scRNA-seq for personalized medicine by determining drug candidates for better cartilage tissue engineering utilizing predicting computational analysis.
No datasets are available for this submission.
No license information is available for this submission.