We use cookies to ensure that we give you the best experience on our website Learn more

Home

Saved research

Submission

0149 - Single Cell RNA Sequencing Of Human MSC Chondrogenesis Reveals Multiple Cell Subpopulations And Intermediate Differentiation States As Targets For Drug Discovery

Submitted

and 1 other(s)

30 Views
0 Downloads
0 Saves

Presented at

ORS 2019 Annual Meeting

-

Presentation

thumbnail

Abstract

Bone marrow derived mesenchymal stem cells (MSCs) exhibit the potential to undergo chondrogenesis in vitro, forming de novo tissues with a cartilage-like extracellular matrix that is rich in glycosaminoglycan and collagen type II. However, it is commonly accepted that MSCs comprise an inhomogeneous population of cells, and the fate of individual subpopulations during this differentiation process is not well understood. While changes in the transcriptomic profiles of MSC chondrogenesis had been profiled through bulk analyses of pellet samples, the impact of clonal architecture remains poorly understood. Moreover, strategies targeting the molecularly distinct subpopulations of that drive the clonal structure of MSC chondrogenesis are largely nascent. To address this problem, we have employed single-cell RNA sequencing (scRNA-seq) to dissect the clonal sub-structure of pellets derived from MSC chondrogenesis. As a proof of concept, we highlighted the potentials of scRNA-seq for personalized medicine by determining drug candidates for better cartilage tissue engineering utilizing predicting computational analysis.

Datasets

No datasets are available for this submission.

License

No license information is available for this submission.

Morressier

Company

Legal

Follow us

© Copyright 2020 Morressier GmbH. All rights reserved.

Morressier

© Copyright 2020 Morressier GmbH.
All rights reserved.