Helena Piedade Farsoni
Anderson Jones Bobko
Luciano Flávio Neves Ramos
Celso Luis Marino
Brazil is a world reference in eucalyptus production due to its action based on sustainability, competitiveness and innovation. Accordingly, factors that affect production, such as diseases caused by pathogenic biotic agents, including those of fungal and bacterial origin, need to be studied. Bacteria encompass a group of pathogens of complex morphological differentiation, requiring studies to characterize them. Among the methods for separating bacterial groups, the biochemical characterization allows to distinguish these according to their characteristics, such as types of respiration, production of specific enzymes or carbohydrates, among others. Thus, 39 pathogenic isolates obtained through eucalyptus seedlings with symptoms of die-back and wilting and lesions on the branches, petiole and midrib were evaluated biochemically, in order to evaluate the pathogen group. Consequently, these isolates were submitted to the tests proposed by Schaad (2001), such as potassium hydroxide or Gram test (positive or negative), oxidation and fermentation test, hypersensitivity to tobacco (Nicotiana tabacum), pectinolytic enzyme production, reductive phosphatase activation, reduction of nitrate to nitrite, production of carbohydrates from mannitol and production of catalase. Therefore, the tests demonstrated the bacterium is Gram negative, that is, pathogenic. In addition, the results of the tests of the oxidation and fermentation, reductive phosphatase, nitrate to nitrite reduction, mannitol and catalase were positive and for pectinolytic enzyme production and hypersensitivity to tobacco were negative, indicating the isolates belong to the group of the Enterobacteria.
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