We use cookies to ensure that we give you the best experience on our website Learn more

May 14, 2019

87th EAS Congress

03 - TRIGLYCERIDE RICH LIPOPROTEINS FROM TYPE 2 DIABETIC PATIENTS ACTIVATE PLATELETS

;

Lipoproteins

atherothrombosis

type 2 diabetes

Abstract

Abstract

thumbnail

Keywords

Lipoproteins

atherothrombosis

type 2 diabetes

Abstract

Aim. Type 2 diabetic (T2D) patients have a 2-fold increase risk of developing ischemic cardiovascular ischemic complications driven by atherothrombosis. T2D patients have hyperactive platelets, thus promoting their adhesion to vascular endothelium and their aggregation and hypertriglyceridemia involving large VLDL is a major feature of the diabetic dyslipidemia. Our objective was to determine the effects of Triglyceride Rich Lipoproteins (TGRL) from fasting T2D patients on platelet activation. Methods. TGRL were isolated from the plasma of 25 T2D patients by ultra-centrifugation at a density equivalent to that of chylomicrons (d<1.000). Platelets were isolated from healthy donors, suspended in a Tyrode-HEPES buffer, pre-incubated in the presence or absence of TGRL from T2D patients and stimulated by a subthreshold concentration of agonist. Platelet aggregation and the arachidonic acid (AA) signaling pathway were studied. Results. TGRL fractions (d<1.000) from fasting T2D patients had an ApoB-100/ApoB-48 ratio of 42 ± 7. The triglycerides/total ApoB ratio of 4.2 ± 0.5 and the mean diameter of the particles of 205 ± 16 nm indicated that the TGRL were enriched in triglycerides and large. TGRL significantly potentiated both collagen-stimulated platelet aggregation (+68) and thromboxane B2 (TxB2) concentration (+34%, p<0.001). TGRL significantly increased TxB2 concentration (+37%) through enhanced phosphorylation of p38MAPK and cytosolic phospholipase A2 (+87% and +32% respectively) in platelets co-incubated with T2D TGRL and thrombin compared to platelets incubated with thrombin. Conclusion. TGRL from fasting T2D patients might contribute to atherotrombosis by increasing platelet aggregation via activation of the AA signaling pathway.

Discover over 20,000 new abstracts, posters and presentations from leading academic conferences every month. Stay on top of the latest findings, methodologies and discussions happening in your research field around the world.

Company

Legal

Follow us

© Copyright 2019 Morressier GmbH. All rights reserved.

© Copyright 2019 Morressier GmbH.
All rights reserved.