Dr. E.M. Muralidharan
The fortuitous synchronous flowering of six species of bamboo in the KFRI Bambusetum at Thrissur, India and vicinity during 2014 to 2017 offered the rare window of opportunity to study the phenomenon and exploit the benefits of hybridization. Clumps of Dendrocalamus asper, D. giganteus, P. stocksii, D. strictus and Ochlandra travancorica in the bambusetum and a natural population of Bambusa bambos flowered during the same time in the district. Floral biological features of the species such as anther dehiscence, pollen production, pollen viability and pollen germination were compared. Pollen viability varied from 35 % in P. stocksii to 83% in D. giganteus and germination from 22% in P. stocksii to 78% in D. asper. Hybridization was attempted through reciprocal crosses between the different species. Attempts to improve the anther dehiscence was carried out employing direct sunlight, silica gel, calcium chloride etc. Hurdles in cross pollination were overcome using procedures like use of germination media on the stigma, cut-style pollination, in ovulo fertilization and in vitro culture excised pistils and vitro pollination/fertilization in D. asper, D. giganteus and P. stocksii. Surface sterilization procedures without compromising the viability of the pollen and ovules were developed. Pollen stored in organic solvents survived upto 280 days at 4°C. The precise nutritional and environmental conditions essential to maintain the female receptivity and seed set, time of excising the ovule was determined. Valuable information on pollen storage and in vitro conditions conducive to carrying out hybridization was generated that can facilitate future hybridization efforts.
No datasets are available for this submission.